One minute to introduce MOPSO buffer to you

In 1960, Good's zwitterionic buffer was a buffer system developed by the Good team specifically for life science research, and MOPSO buffer was included in it.

MOPSO, also known as 3-morpholin-2-hydroxypropanesulfonic acid in Chinese, like other buffering agents of the same type, does not interfere with biochemical reaction processes, especially without significant inhibitory effects on enzyme chemical reactions. Therefore, it is specifically used for the study of organelles, pH sensitive proteins, and enzymes.  

Characteristics of MOPSO:

MOPSO has high solubility in water and does not precipitate with metal ions. Its pKa is between 6 and 8, and the salt effect is very small. In addition, it is not easily affected by factors such as temperature, ion composition, and concentration; It will not interfere with biological activity and has stable chemical properties.  

MOPSO configuration method:

If 1 liter of solution is required, 800 mL of distilled water should be prepared in a beaker. 112.63 grams of MOPSO powder should be added to the beaker containing the distilled water, and adjusted to the desired pH range with 10% sodium hydroxide solution. Distilled water should be added until the required volume is reached, and the preparation is complete.

Application of MOPSO:

MOPSO can be used to prepare stable bottom solutions in low-temperature biochemical working environments; Used as a buffer system for determining by-products of bio marine crude oil, it can also be used in carrier electrolytes and new liquid phase separation technologies such as capillary electrophoresis. It can also be used for buffering the preparation of glutathione synthase crystals and some physicochemical research methods, such as spectrophotometry.

In addition, the peptide backbone of MOPSO and BSA also interact with each other, which can to some extent prevent BSA from undergoing thermal denaturation, and it can serve as a buffer for copper related analysis.

Hubei Xindesheng Material Technology Co., Ltd. is the source manufacturer of biological buffering agents. It not only develops and produces MOPSO buffering agents, but also Tris, Tris HCL, BICINE, CAPS, MOPS and other buffering agents, with a complete range of categories. With 17 years of development, we have rich experience and standard pre-sales and after-sales services to ensure product quality and customer satisfaction. If you have purchasing needs, you can visit the official website of Desheng Company for more information or directly call to place an order. Desheng can provide one-stop procurement services and welcomes your purchase!  

Tags: MOPSO buffer, 68399-77-9, Biological buffer