The use of 3- [N, N-di (hydroxyethyl) amino] -2-hydroxypropanesulfonic acid DIPSO

3- [N, N-di (hydroxyethyl) amino] -2-hydroxypropanesulfonic acid, commonly referred to as DIPSO, is a commonly used biochemical experimental buffer widely used in scientific research and chemical engineering. The properties of its white crystalline powder determine its unique applications in solubility, acid-base regulation, and other aspects.

1、 Solubility and acid-base regulation

The solubility of DIPSO is very low, almost insoluble in organic solvents involved in biochemical reactions. In an aqueous solution at 20 ℃, the saturation is only 0.1M, which gives DIPSO a certain advantage in preparing buffer solutions. By dissolving DIPSO in water, a colorless and transparent solution can be obtained, with a pH value in the acidic range, making it suitable for adjusting the acidity and alkalinity of biochemical experimental systems. In addition, using the dissolved solution as a buffer can effectively maintain the pH value of the solution within the range of 7.0-8.2, providing a stable reaction environment for the experiment.

2、 Application in Biochemical Separation and Purification

DIPSO plays an important role in chromatographic separation and purification of proteins. In these experiments, maintaining pH stability is crucial for the accuracy and efficiency of the separation process. The use of DIPSO buffer can ensure the stability of pH value during the separation process, avoiding the impact of pH changes on the separation efficiency of ionizable compounds, which is crucial for protein separation and purification.

3、 Application in electrophoresis experiments

DIPSO buffer has also been widely used in electrophoresis technology. The change in pH value of the electrophoretic solution may affect the accuracy of the experimental results when current passes through the experimental substance. The use of DIPSO buffer can maintain the pH stability of the solution, provide the required ions for electrophoresis experiments, and ensure the stability and reproducibility of the migration process.

4、 Combination application of multiple buffering agents

In biochemical research, different experiments require different buffer systems and pH adjustment ranges. The use of a single buffer may limit the adjustment of pH range. Therefore, DIPSO is often combined with other buffers such as HEPES, MOPS, etc. to form new buffers. This combination can expand the pH high activity range of the buffer, thereby reducing the required buffer concentration and minimizing the potential toxic effects of the buffer on biochemical substances.

DIPSO buffer has various applications in biochemical experiments due to its unique solubility and acid-base regulation properties. From separation and purification to electrophoresis experiments, it has provided important support for research and experimentation in the field of biochemistry.

As a manufacturer of DIPSO, Desheng is committed to providing high-quality DIPSO raw materials to meet the diverse needs of biochemical research and experimentation. We have advanced production equipment and strict quality control system, conducting multiple inspections to ensure the stability and purity of each batch of products. We will provide professional support and solutions for customers in terms of product selection, usage methods, and technical consultation. If you have any relevant intentions, please click to consult. Desheng is definitely your trusted partner.

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