Advantages of N - (2-acetylamino) -2-iminodiacetic acid (ADA) buffer

Buffers play a crucial role in chemical and biological experiments, as they can stabilize the pH value of solutions, thereby maintaining the stability and accuracy of reaction systems. Among numerous buffering agents, N - (2-acetamido) -2-iminodiacetic acid (ADA) has attracted much attention due to its unique properties and wide range of applications. What are its advantages that can attract so much attention from everyone? Let's take a look together below.

1、 Stability

ADA buffer, as an amino acid buffer, has excellent stability. It can maintain the stability of the solution within the neutral and weakly acidic pH range, unaffected by temperature changes and ion concentration. Compared to other buffering agents, ADA is less prone to decomposition during long-term storage and use, ensuring the accuracy and reliability of experimental results.

2、 Wide pH adjustment range

ADA buffer has a wide pH adjustment range, with a pH value of 1.5-3.0 in aqueous solution, indicating acidity. When added to the experimental solution, its high activity range can be between 5.8 and 7.4, suitable for various experimental needs. In biological research, many enzymes and proteins have pH values that fall within the regulatory range of ADA buffer, making it an advantageous buffer for many biological experiments.

3、 Coordination ability with metal ions

ADA buffer not only effectively stabilizes the pH value of the solution, but also has good metal ion coordination ability. In some experiments, specific metal ions may interfere with the reaction, and the synergistic effect of ADA buffer can stabilize these metal ions, thereby avoiding interference and keeping the experiment undisturbed.

4、 Application in biological experiments

It has a wide range of applications in biological experiments, especially in the fields of molecular biology and biochemistry. In experiments, to prevent protein oxidation, ADA can be added to prevent irreversible reactions. Meanwhile, due to its low dependence on proteins, ADA can also be used in cell culture media. Even in the absence of proteins, ADA can support the survival of CEF cells and maintain the reproducibility of experiments.

N - (2-acetylamino) -2-iminodiacetic acid (ADA) buffer is highly favored due to its stability, wide pH adjustment range, and ability to coordinate with metal ions. As a high-quality buffering agent, it provides strong guarantees for the successful conduct of experiments and the accuracy of results. As a leading producer of ADA buffering agents, Desheng can provide raw materials with a purity of up to 99%, good water solubility, simple preparation, and is suitable for multiple experimental fields. If you are interested, please feel free to contact us at any time to make a purchase!

Tags: ADA buffer, 26239-55-4, Biological buffer